Protein Cutter
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Sequence string
Import from file
(*.fasta, *.txt)
Visualizer
Gly
Ala
Val
Leu
Ile
Met
Pro
Phe
Trp
Ser
Asn
Gln
Thr
Tyr
Cys
Lys
Arg
His
Asp
Glu
Statistics
Options
ionization mode
[M+H]
[M]
[M-H]
Cys modification (fixed)
none
Carbamidomethyl-C (Cys_CAM)
Carboxymethyl-C (Cys_CM)
Met oxidation (variable)
missed cleavage
0
1
2
3
4
Cutting rules
cut by enzyme
none (set below)
Trypsin (after KR)
Arg-C (after R)
Asp-N (before BD)
Asp-N_ambic (before DE)
Chymotrypsin (after FLWY)
CNBr (after M)
CNBr+Trypsin (after MRK)
Formic_acid (before D, after D)
Lys-C (after K)
Lys-C/P (after K)
PepsinA (after FL)
Tryp-CNBr (after KMR)
TrypChymo (after FKLRWY)
Trypsin/P (after KR)
V8-DE (after BDEZ)
V8-E (after EZ)
semiTrypsin (after KR)
LysC+AspN (before BD, after K)
Prolyl_endoprotease (after PA)
add new cutting rule
before
after
between
defined rules
Setting-up filters
Mono.Mass
none
more than
less than
more than - less than
Avg.Mass
none
more than
less than
more than - less than
Length
none
more than
less than
more than - less than
Hydropathicity
none
more than
less than
more than - less than
NPS
none
more than
less than
more than - less than
Isoel.point
none
more than
less than
more than - less than